Table 2-1C: AOAC Official Method 985.15

AOAC Official Method 985.15 (A3)

Fat (Crude) in Meat and Poultry Products

Rapid Microwave-Solvent Extraction Method

Type

Matrix

Analytes

Detection

Control

Microwave

Drying

with

Non-Microwave Solvent Extraction

Meat

Poultry

Fat

Balance

NA

Condensed Procedure

Initial Microwave Drying:

Prepare sample as in Method 983.18.

Place two glass fiber pads on balance and tare.

Rapidly and evenly spread ~4 g onto one glass pad.

Cover sample with second pad.

Determine initial mass.

Microwave heat for 3 - 5 min. at 80 - 100 % depending on product type.

Non-Microwave Extraction:

Place Pads into non-microwave automated solvent extractor

Extract with CH2Cl2

Final Microwave Drying

Place Pads on balance and tare

Microwave heat for 30 sec. at 80 - 100%

Determine final mass and calculate % moisture.

Repeat drying until constant weight is obtained

Determine % Fat

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