Appendix 1. Biological Reference Materials

Appendix 1.2 Botanical
Matrix	Analytes	Reagents	Cavity/Vessel	Conditions	Detection	Ref.
BCR Aquatic plant 60	Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Pb, Zn	HNO3, HCl	Multimode/ LP closed	15 min at 300 W, 5 min at 0 W, 20 min at 510 W	F-AAS ETV-AAS F-OES	E72
	Ni	HNO3, HCl	Multimode/ HP closed	4 min at 350 W, 10 min at 140 W	ETV-AAS	E9
	Fe	A. HNO3 B. 1. HNO3 2. HF	Multimode/ HP closed Multimode/ HP closed	30 sec at 700 W Same as A	ETV-AAS	E6
	Cd, Cu, Pb	1. HNO3, H2O, H2O2 2. Aqua regia, HF (optional)	Multimode/ open	1. 15 min at 70 W, 15 min at 150 W, 15 min at 180 W, 1 min at 250 W, 1 min at 600 W 2. 15 min at 70 W, 1 min at 600 W	ETV-AAS	E5
BCR Aquatic moss 61 (contd.)	Cd	HNO3, HCl	Multimode/ HP closed	4 min at 360 W, 10 min at 180 W	ETV-AAS	E4
	Cu, Cd, Mn, Pb, Zn	HNO3	Multimode/ HP closed	30 sec at 665 W	F-OES F-AAS ICP-OES	E49
	Ni	HNO3, HCl	Multimode/ HP closed	4 min at 360 W, 10 min at 180 W	ICP-OES	E10, E11
BCR Aquatic moss 61	Cu, Cd, Mn, Pb, Zn	HNO3	Multimode/ HP closed	30 sec at 665 W	F-OES F-AAS ICP-OES	E49
	Fe	A. HNO3 B. 1. HNO3 2. HF	Multimode/ HP closed Multimode/ HP closed	30 sec at 700 W Same as A	ETV-AAS	E6
BCR Aquatic moss 61 (contd.)	Cd	HNO3, HCl	Multimode/ HP closed	4 min at 360 W, 10 min at 180 W	ETV-AAS	E4
	Ni	HNO3, HCl	Multimode/ HP closed	4 min at 350 W, 10 min at 140 W	ETV-AAS	E9
	Ni	HNO3, HCl	Multimode/ HP closed	4 min at 360 W, 10 min at 180 W	ICP-OES	E10, E11
BCR Olive blossom 62	Cu, Cd, Mn, Pb, Zn	HNO3	Multimode/ HP closed	30 sec at 665 W	F-OES F-AAS ICP-OES	E49
	Ni	HNO3, HCl	Multimode/ HP closed	4 min at 350 W, 10 min at 140 W	ETV-AAS	E9
	Ni	HNO3, HCl	Multimode/ HP closed	4 min at 360 W, 10 min at 180 W	ICP-OES	E10, E11
	Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Pb, Zn	HNO3, HCl	Multimode/ LP closed	15 min at 300 W, 5 min at 0 W, 20 min at 510 W	F-AAS ETV-AAS F-OES	E72
	Cd, Cu, Mn, Pb	1. HNO3, H2O, H2O2 2. Aqua regia, HF (optional)	Multimode/ open	1. 15 min at 70 W, 15 min at 150 W, 15 min at 180 W, 1 min at 250 W, 1 min at 600 W 2. 15 min at 70 W, 1 min at 600 W	ETV-AAS	E5
	Pb	HNO3, HCl	Multimode/ flow through	18 sec at 700 W (0.4 mL coil, 1.5 mL min-1)	ETV-AAS	E8
	Cu, Mg, Pb	HNO3	Multimode/ LP closed	3 min at 650 W	ETV-AAS	E3
BCR Spruce needles 101	Cd, Cu, Pb	HNO3, HClO4, H2SO4	Multimode/ HP closed.	10 min at 85 bar	DP-ASV	E1
	Al, Ca, Mg, Mn, P, Zn	1. HNO3 2. HNO3 3. H2O2 4. H2O	Single mode/ open	1. 5 min at 10 W, 10 min at 30 W, 10 min at 60 W 2. 10 min 60 W 3. 5 min at 60 W 4. 5 min at 50 W	ICP-OES ICP-MS HPLC-ICP-MS	E13
BCR Wholemeal flour 189	Cd, Cu, Fe, Mn, Zn	A. HNO3, H2O2 B. HNO3, H2O2 C. HNO3, HClO4	Multimode/ HP closed Multimode/ HP closed Multimode/ HP closed	75 sec at 665 W 1 min at 250 W, 2 min at 0 W, 2 min at 250 W, 2 min at 400 W, 2 min at 600 W 150 sec at 950 W, 60 sec at 0 W, 90 sec at 300 W, 90 sec at 500 W, 90 sec at 700 W, 90 sec at 850 W	ETV-AAS	E6
BCR Ulva lactuca 279	Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Pb, Zn	HNO3	Multimode/ LP closed	5 min at 300 W, 5 min at 0 W, 5 min at 300 W, 5 min at 450 W, 5 min at 0 W, 5 min at 450 W	F-AAS ETV-AAS F-OES	E72
BCR White clover 402	Co, Mo	A. 1. HNO3 2. HNO3 3. H2O2 4. H2O B. 1. HNO3, HCl 2. HNO3, HCl 3. H2O2 4. H2O	Single mode/ open Single mode/ open	1. 5 min at 10 W, 10 min at 30 W, 10 min at 60 W 2. 10 min 60 W 3. 5 min at 60 W 4. 5 min at 50 W 1. 5 min at 40 W, 10 min at 50 W 2. 20 min 54 W 3. 5 min at 40 W 4. 5 min at 50 W	ICP-OES ICP-MS HPLC-ICP-MS	E13
IAEA Hay powder V10	Mo	1. HNO3 2. Perhydrol	Multimode/ open then Multimode/ LP closed	1. 30 min at 180W (open), 10 min at 420W, 10 min at 600W 2. 10 min at 600W	MS	E73
	I	HNO3, N2H4	Multimode/ HP closed	35 sec at 675 W	NAA	E17
IAEA Hay powder V10 (contd.)	Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Pb, Zn	HNO3, HCl	Multimode/ LP closed	15 min at 300 W, 5 min at 0 W, 20 min at 510 W	F-AAS ETV-AAS F-OES	E72
	Hg	1. HNO3 2. H2O2	Multimode/ HP closed	3 min at 450 W	Radiochemical	E30
	Ag, Ba, Bi, Cd, Co, Cr, Cs, Cu, Ga, Hg, Li, Mo, Ni, Pb, Rb, Sn, Sr, Th, U, Zn, Zr	HNO3	Multimode/ HP closed	1 min at 300 W (x3-5), 7 min at 300 W, 2 min at 600 W	ICP/MS	E74
MOE Vegetation V85-1	Al, Ba, Ca, Cu, Fe, Mg, Mn, Zn	Aqua regia	Multimode/ stopped flow	2 min at 720 W or 32 min at 720 W	ICP-OES	E58
MOE Norway maple	Al, Ba, Ca, Cu, Fe, Mg, Mn, Pb, Zn	A. Aqua regia B. Aqua regia	Multimode/ stopped flow Multimode/ open	2 min at 720 W 2 min at 720 W, 2 min at 0 W, 2 min at 720 W, 2 min at 0 W, 2 min at 720 W (with water load)	ICP-OES	E58
MOE White birch	Al, Ba, Ca, Cd, Cu, Fe, Mg, Mn, Zn	A. Aqua regia B. Aqua regia	Multimode/ stopped flow Multimode/ open	2 min at 720 W 2 min at 720 W, 2 min at 0 W, 2 min at 720 W, 2 min at 0 W, 2 min at 720 W (with water load)	ICP-OES	E58
NIES Pepperbush 1	Ca, Fe, Mg, Zn	HNO3	Multimode/ flow through	100 sec at 525 W (10 mL coil, 6 mL min-1)	F-AAS	E39
	Ca, Cu, Fe, Mg, Mn, Zn	HNO3, HClO4, HCl, HF	Multimode/ MP closed	14 min intermittent 200 W	F-AAS	E27
NIES Pepperbush 1 (contd.)	Co	HNO3, HCl	Multimode/ MP closed	5 min at 600 W (50 mL water load), 5 min at 600 W (no load)	UV-Vis	E75
	Cd, Co, Cu, Ni, Pb	HNO3, HCl, HClO4, HF	Multimode/ HP closed	5 min at 200 W (water load), 3.5 min at 200 W (no load)	ICP-OES ETV-ICP	E28
	Cd, Cu, Fe, Mn, Zn	A. HNO3, H2O2 B. HNO3, H2O2 C. HNO3, HClO4	Multimode/ HP closed Multimode/ HP closed Multimode/ HP closed	75 sec at 665 W 1 min at 250 W, 2 min at 0 W, 2 min at 250 W, 2 min at 400 W, 2 min at 600 W 150 sec at 950 W, 60 sec at 0 W, 90 sec at 300 W, 90 sec at 500 W, 90 sec at 700 W, 90 sec at 850 W	ETV-AAS	E6
	Fe, Mn, Zn	HNO3	Multimode/ flow through	-	F-AAS ICP-OES ICP/MS	E66
	Cd	1. HNO3, HClO4, HCl, HF 2. HClO4	Multimode/ MP closed	1. 5 min at 200 W (using water load), 4 min at 200 W (no load)	F-AAS	E76
NIES Tea leaves 7	Ca, Cu, Fe, Mg, Mn, Zn	HNO3, HClO4, HCl, HF	Multimode/ MP closed	14 min intermittent 200 W	F-AAS	E27
	Co	HNO3, HCl	Multimode/ LP closed	5 min at 600 W (50 mL water load), 5 min at 600 W (no load)	UV-Vis	E75
	Cd, Co, Cu, Ni, Pb	HNO3, HCl, HClO4, HF	Multimode/ MP closed	5 min at 200 W (water load), 3.5 min at 200 W (no load)	ICP-OES ETV-ICP	E28
NIES Sargasso 9	Ca, Fe, Mg, Zn	HNO3	Multimode/ flow through	100 sec at 525 W (10 mL coil, 6 mL min-1)	F-AAS	E39
	Fe, Mn, Zn	HNO3	Multimode/ flow through	-	F-AAS ICP-OES ICP/MS	E66
	As, Cr, V	HNO3, H2O2	Multimode/ LP closed	3 min at 300 W	ICP/MS	E77
	Ca, Cu, Fe, Mg, Mn, Zn	HNO3, HClO4, HCl, HF	Multimode/ MP closed	14 min intermittent 200 W	F-AAS	E27
NIES Rice flour 10	Mo	1. HNO3 2. Perhydrol	Multimode/ open then Multimode/ LP closed	1. 30 min at 180W (open), 10 min at 420W, 10 min at 600W 2. 10 min at 600W	MS	E73
	Cd	1. HNO3, HClO4, HCl, HF 2. HClO4	Multimode/ MP closed	1. 5 min at 200 W (using load), 4 min at 200 W (no load)	F-AAS	E76
NIST Apple leaves 1515	Ba, Ce, Co, Cr, Eu, Fe, Hf, K, La, Na, Sb, Sc, Sm, Sr, Tb, Zn	1. HNO3, HF 2. HClO4	Multimode/ LP closed	1. 20 min at 373 W (9 vessels) 2. 20 min at 545 W (6 vessels)	NAA	E78
NIST Peach leaves 1547	Ba, Ce, Co, Cr, Eu, Fe, Hf, K, La, Na, Sb, Sc, Sm, Sr, Tb, Zn	1. HNO3, HF 2. HClO4	Multimode/ LP closed	1. 20 min at 373 W (9 vessels) 2. 20 min at 545 W (6 vessels)	NAA	E78
NIST Wheat flour 1567	Mo	1. HNO3 2. Perhydrol	Multimode/ open then Multimode/ LP closed	1. 30 min at 180 W (open), 10 min at 420 W, 10 min at 600 W 2. 10 min at 600W	MS	E73
	Se	1. HNO3 2. H2SO4, HClO4 3. H2O	Single mode/ open	1. 15 min at 45 W, 10 min at 75 W 2. 10 min at 90 W, 35 min at 120 W 3. 8 min at 120 W	ETV-AAS	E18
	Cd	HNO3	Multimode/ LP closed	2 min on defrost three times	ETV-AAS	E40
	Hg	HNO3	Multimode/ LP closed	8 min at 140 W, 8 min at 280 W, 4 min at 420 W	CV-AAS	E2
	Ba, Ca, K, Mg, Mn, Na, P, S, Zn	1. HNO3 2. H2O2	Multimode/ open	1. 30 min at 540 W 2. 30 min at 540 W	ICP-OES	E42
	Residual amino acids	HNO3	Multimode/ LP closed	Varied	Fluorimetric	E43
NIST Wheat flour 1567 (contd.)	Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn	A. 1. HNO3 2. H2O2 3. H2O2 B. 1. HNO3 2. H2O2, HF 3. H3BO3	Multimode/ open Multimode/ open (with reflux top)	1. 5 min at 300 W, 600 W until 1 mL remains 2. 30-40 min at 600 W until 1 mL remains 1. 5 min at 300 W, 20-30 min at 600 W reflux not on 2. 30 min at 180 W reflux on 3. 180 W reflux on	FI-ICP-OES	E35
	Se	HNO3	Multimode/ LP closed	(1.5 h at 60°C on hotplate) 4 min at 287 W	ETV-AAS	E44
	Ca, Cu, Fe, K, Mn, Zn	HNO3	Multimode/ HP closed	30 sec at 665 W	F-OES F-AAS ICP-OES	E49
	As, Ca, Cu, Fe, Mg, Mn, Mo, P, S, Se, U, V, Zn	HNO3, H2O2	Multimode/ MP closed (Max 350 psi - power cutoff at 150 psi)	4 min at 296 W, 8 min at 360 W	F-AAS ICP/MS ICP-OES	E79
	Cu, Fe, K, Mn, Zn	HNO3	Multimode/ LP closed	8 min at 150 W	F-AAS ETV-AAS SIMAAC	E54
	Ca, Cu, Fe, K, Mg, Mn, P, Zn	HNO3, HCl	Multimode/ LP closed	5 min at 300 W, 5 min at 0 W, 5 min at 300 W, 5 min at 450 W, 5 min at 0 W, 5 min at 450 W	ICP-OES	E80
NIST Wheat flour 1567 (contd.)	Cd, Cu, Fe, Mn	1. HNO3, H2O, H2O2 2. Aqua regia, HF (optional)	Multimode/ open	1. 15 min at 70 W, 15 min at 150 W, 15 min at 180 W, 1 min at 250 W, 1 min at 600 W 2. 15 min at 70 W, 1 min at 600 W	ETV-AAS	E5
	Ca, Fe, K, Mg, Mn, Na, P, S	1. HNO3 2. H2O2 3. HCl	Multimode/ LP closed	1. 30 min at 540 W, 5 min at 0 W 2. 5 min at 0 W, 15 min at 540 W, 5 min at 0 W 3. 10 min at 180 W	ICP-OES	E81
NIST Rice flour 1568	Ba, Ca, K, Mg, Mn, Na, P, S, Zn	1. HNO3 2. H2O2	Multimode/ open	1. 30 min at 540 W 2. 30 min at 540 W	ICP-OES	E42
	Residual amino acids	HNO3	Multimode/ LP closed	Varied	Fluorimetry	E43
	Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn	A. 1. HNO3 2. H2O2 3. H2O2 B. 1. HNO3 2. H2O2, HF 3. H3BO3	Multimode/ open Multimode/ open (with reflux top)	1. 5 min at 300 W, 600 W until 1 mL remains 2. 30-40 min at 600 W until 1 mL remains 1. 5 min at 300 W, 20-30 min at 600 W reflux not on 2. 30 min at 180 W reflux on 3. 180 W reflux on	FI-ICP-OES	E35
	B	HNO3, H2O2	Multimode/ HP closed	2 min at 100W, 2 min at 0 W, 5 min at 250 W, 3 min at 0 W, 5 min at 500 W, 15 min at 250 W	UV-Vis Fluorimetry ICP-OES ICP/MS	E64
	As, Ca, Cu, Fe, Mg, Mn, Mo, P, S, Se, U, V, Zn	HNO3, H2O2	Multimode/ MP closed (Max 350 psi - power cutoff at 150 psi)	4 min at 296 W, 8 min at 360 W	F-AAS ICP/MS ICP-OES	E79
	Cr, Hg, Se	1. HNO3 2. H2O2	Multimode/ HP closed	1. 3 min at 450 W	Radiochemical	E30
NIST Spinach 1570	I	HNO3, N2H4	Multimode/ HP closed	35 sec at 675 W	NAA	E17
	Ba, Ca, K, Mg, Mn, Na, P, S, Zn	1. HNO3 2. H2O2	Multimode/ open	1. 30 min at 540 W 2. 30 min at 540 W	ICP-OES	E42
NIST Spinach 1570 (contd.)	Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn	A. 1. HNO3 2. H2O2 3. H2O2 B. 1. HNO3 2. H2O2, HF 3. H3BO3	Multimode/ open Multimode/ open (with reflux top)	1. 5 min at 300 W, 600 W until 1 mL remains 2. 30-40 min at 600 W until 1 mL remains 1. 5 min at 300 W, 20-30 min at 600 W reflux not on 2. 30 min at 180 W reflux on 3. 180 W reflux on	FI-ICP-OES	E35
	Al, Ba, Ca, Cr, Cu, Fe, K, Li, Mg, Mn, Na, P, Si, Sr, Ti, Zn	1. Aqua regia 2. HBO3	Multimode/ MP closed (evacuated slightly at start)	1. 3 min at 625 W	ICP-OES	E55
NIST Orchard leaves 1571	I	HNO3, N2H4	Multimode/ HP closed	35 sec at 675 W	NAA	E17
	Cd	HNO3	Multimode/ LP closed	2 min on defrost three times	ETV-AAS	E40
	As	HNO3, HClO4, H2SO4	Multimode/ HP closed.	10 min at 85 bar	DP-ASV	E1
	Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn	A. 1. HNO3 2. H2O2 3. H2O2 B. 1. HNO3 2. H2O2, HF 3. H3BO3	Multimode/ open Multimode/ open (with reflux top)	1. 5 min at 300 W, 600 W until 1 mL remains 2. 30-40 min at 600 W until 1 mL remains 1. 5 min at 300 W, 20-30 min at 600 W reflux not on 2. 30 min at 180 W reflux on 3. 180 W reflux on	FI-ICP-OES	E35
	As, Co, Cr, Cu, Ni, Pb, Se, Zn	HNO3, HClO4	Multimode/ open	600 W until first signs of HClO4 fumes	F-AAS NAA	E46, E47, E48
NIST Orchard leaves 1571 (contd.)	Cd	1. HNO3, HClO4, HCl, HF 2. HClO4	Multimode/ MP closed	1. 5 min at 200 W (using water load), 4 min at 200 W (no load)	F-AAS	E76
	Al, Ba, Ca, Cr, Co, Cu, Fe, K, Mg, Mn, Na, P , S, Zn	HNO3	Multimode/ LP closed	10 min at 70 psi, 15 min at 450 W	NAA ICP-OES	E22
	Po	HNO3	Multimode/ MP closed	60 min at 60 W, cool 60 min at 60 W	a-spectrometry	E82
	Cd, Co, Fe, Ni, Pb	HNO3, HF	Multimode/ LP closed	1 min at 240 W, cool (x15)	ETV-AAS X-ray Fluorescence	E83
	Al, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, P, Zn	1. Aqua regia 2. HBO3	Multimode/ MP closed (evacuated slightly at start)	3 min at 625 W	ICP-OES	E55
	Se	HNO3, HClO4	Not reported	-	Fluorimetry	E65
	Cu, Pb, Zn	HNO3, HClO4	Multimode/ open	3 min at 600 W	ETV-AAS	E84
	Al, Ba, Ca, Cu, Fe, Mg, Pb, Zn	A. Aqua regia B. Aqua regia	Multimode/ stopped flow Multimode/ open	2 min at 720 W 2 min at 720 W, 2 min at 0 W, 2 min at 720 W, 2 min at 0 W, 2 min at 720 W (with water load)	ICP-OES	E58
	Al	HNO3	Multimode/ HP closed	1 min at 150 W, 30 min at 0 W, 1 min at 450 W	ICP-OES	E20
NIST Citrus leaves 1572	Al, Ag, As, Ba, Ca, Cl, Cr, Cu, Fe, K, Li, Mg, Mn, Mo, N, Na, Ni, P, Pb, Rb, S, Sb, Si, Sr, Ti, Zn	HNO3, HClO4	Multimode/ MP closed	15 min at 0 W, 10 min at 150 W, 10 min at 450 W, 15 min at 0 W	ICP-OES	E31
	I	HNO3, N2H4	Multimode/ HP closed	35 sec at 675 W	NAA	E17
	Cd	HNO3	Multimode/ LP closed	2 min on defrost three times	ETV-AAS	E40
	Ba, Ca, K, Mg, Mn, Na, P, S, Zn	1. HNO3 2. H2O2	Multimode/ open	1. 30 min at 540 W 2. 30 min at 540 W	ICP-OES	E42
NIST Citrus leaves 1572 (contd.)	Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn	A. 1. HNO3 2. H2O2 3. H2O2 B. 1. HNO3 2. H2O2, HF 3. H3BO3	Multimode/ open Multimode/ open (with reflux top)	1. 5 min at 300 W, 600 W until 1 mL remains 2. 30-40 min at 600 W until 1 mL remains 1. 5 min at 300 W, 20-30 min at 600 W reflux not on 2. 30 min at 180 W reflux on 3. 180 W reflux on	FI-ICP-OES	E35
	-	HNO3	Multimode/ LP closed	10 min at 360 W, 5 min at 480 W	-	E85
	Cd	1. HNO3, HClO4, HCl, HF 2. HClO4	Multimode/ MP closed	1. 5 min at 200 W (using water load), 4 min at 200 W (no load)	F-AAS	E76
	Hg	HNO3	Multimode/ LP closed	800 W until 75°C, 3 min at 75°C	CV-FANES	E86
	Hg	HNO3	Multimode/ HP closed	90 sec at 600 W (estimated power)	CV-AAS	E87
	Al	HNO3	Multimode/ MP closed	4 min at 200 W, cool, 4 min at 350 W, cool, 8 min at 250 W, cool, 10 min at 400 W (x4)	ETV-AAS	E88
	Sr	HNO3	Multimode/ open	Heat until dryness.	ID (stable isotope dilution activation analysis)	E89
	As, Ca, Cu, Fe, Mg, Mn, Mo, P, S, Se, U, V, Zn	HNO3, H2O2	Multimode/ MP closed (Max 350 psi - power cutoff at 150 psi)	4 min at 296 W, 8 min at 360 W	F-AAS ICP/MS ICP-OES	E79
NIST Citrus leaves 1572 (contd.)	Ca, Cu, Fe, K, Mg, Mn, P, Zn	HNO3, HCl	Multimode/ LP closed	5 min at 300 W, 5 min at 0 W, 5 min at 300 W, 5 min at 450 W, 5 min at 0 W, 5 min at 450 W	ICP-OES	E80
	As, Cr, Hg, Sb	1. HNO3 2. H2O2	Multimode/ HP closed	3 min at 450 W	Radiochemical	E30
	Ba, Ca, Cu, Mg, Mn, Zn	1. HNO3, HF 2. HBO3	Multimode/ LP closed	2 min at 240 W, 2 min 360 W, 16 min at 160°C (600W)	ICP-OES	E90
	Ni	HNO3, HCl	Multimode/ HP closed	4 min at 350 W, 10 min at 140 W	ETV-AAS	E9
	Ni	HNO3, HCl	Multimode/ HP closed	4 min at 360 W, 10 min at 180 W	ICP-OES	E10, E11
	Al, Ca, Fe, K, Mg, Mn, Na, P, S	1. HNO3 2. H2O2 3. HCl	Multimode/ LP closed	1. 30 min at 540 W, 5 min at 0 W 2. 5 min at 0 W, 15 min at 540 W, 5 min at 0 W 3. 10 min at 180 W	ICP-OES	E81
	P	1. HNO3, HClO4 2. HClO4	Multimode/ open (with scrubber)	1. 15 min at 750 W	ICP-OES	E91
	Al	HNO3, H2SO4	Multimode/ MP closed	5 min at 1170 W	IC	E92
	As	HNO3, H2O2	Multimode/ HP closed	-	ICP/MS	E93
	Ni	HNO3, HCl	Multimode/ HP closed	4 min at 460 W, 10 min at 180 W (10 mL water load)	ICP-OES	E94
NIST Citrus leaves 1572 (contd.)	Cu, Fe, Mn, Pb	1. HNO3, H2O, H2O2 2. Aqua regia, HF (optional)	Multimode/ open	1. 15 min at 70 W, 15 min at 150 W, 15 min at 180 W, 1 min at 250 W, 1 min at 600 W 2. 15 min at 70 W, 1 min at 600 W	ETV-AAS	E5
	Ca, Fe, K, Mg, Mn, Zn	-	Multimode/ open	30 min at 650 W	F-AAS	E95
	Ca, Cu, Fe, Mg, Mn, K, Na, Zn	1. HNO3 2. HNO3 3. H2O2	Single mode/ open	1. 10 min at 60 W, 5 min at 80 W 2. 5 min at 70 W 3. 3 min at 60 W	F-AAS	E67
	Al	HNO3	Multimode/ HP closed	1 min at 150 W, 30 min at 0 W, 1 min at 450 W	ICP-OES	E20
	Al, As, Ba, Cr, Cu, Fe, K, Mg, Mn, Ni, P, Pb, Rb, Sr, Zn	1. HNO3 2. HF 3. H2O2	Multimode/ HP closed	1. 5 min at 300 W, 2 min at 600 W, 2 min at 0 W, 3 min at 300 W 2. 5 min at 300 W, 2 min at 600 W, 2 min at 0 W 3. 5 min at 300 W, 2 min at 600 W, 2 min at 0 W, 3 min at 300 W	DCP-OES F-AAS	E96
NIST Tomato leaves 1573	Cu, Mn	HNO3, H2O2	Multimode/ flow through	2 min at 650 W (digestate recirculated through oven)	F-AAS	E97
	Ba, Ca, K, Mg, Mn, Na, P, S, Zn	1. HNO3 2. H2O2	Multimode/ open	1. 30 min at 540 W 2. 30 min at 540 W	ICP-OES	E42
	Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn	A. 1. HNO3 2. H2O2 3. H2O2 B. 1. HNO3 2. H2O2, HF 3. H3BO3	Multimode/ open Multimode/ open (with reflux top)	1. 5 min at 300 W, 600 W until 1 mL remains 2. 30-40 min at 600 W until 1 mL remains 1. 5 min at 300 W, 20-30 min at 600 W reflux not on 2. 30 min at 180 W reflux on 3. 180 W reflux on	FI-ICP-OES	E35
	As, Cr, Cu, Fe, Mn, Pb, Rb, Sr, Th, U, Zn	1. HNO3 2. HF	Multimode/ MP closed	1. Leave overnight, 3 min at 130 W, 6 min at 195 W, 6 min at 260 W, 6 min at 325 W, cool, vent 2. 6 min at 325 W	ICP/MS	E98
	Cd	1. HNO3, HClO4, HCl, HF 2. HClO4	Multimode/ MP closed	1. 5 min at 200 W (using water load), 4 min at 200 W (no load)	F-AAS	E76
NIST Tomato leaves 1573 (contd.)	Ca, Cu, Fe, K, Mn, P, Pb, Zn	HNO3	Multimode/ HP closed	30 sec at 665 W	F-OES F-AAS ICP-OES	E49
	Sr	HNO3	Multimode/ open	Heat until dryness.	ID (stable isotope dilution activation analysis)	E89
	Al, Ba, Ca, Cd, Cr, Fe, K, Mg, Mn, Na, P	1. Aqua regia 2. HBO3	Multimode/ MP closed (evacuated slightly at start)	3 min at 625 W	ICP-OES	E55
	Cd, Co, Fe, Ni, Pb	HNO3, HF	Multimode/ LP closed	1 min at 240 W, cool (x15)	ETV-AAS X-ray Fluorescence	E83
	Cr, Hg, Se	1. HNO3 2. H2O2	Multimode/ HP closed	3 min at 450 W	Radiochemical	E30
	Fe	A. HNO3 B. 1. HNO3 2. HF	Multimode/ HP closed Multimode/ HP closed	30 sec at 700 W Same as A	ETV-AAS	E6
	Ca, Fe, K, Mg, Mn, Zn	-	Multimode/ open	30 min at 650 W	F-AAS	E95
	B	HNO3, H2O2	Multimode/ HP closed	2 min at 100W, 2 min at 0 W, 5 min at 250 W, 3 min at 0 W, 5 min at 500 W, 15 min at 250 W	UV-Vis Fluorimetry ICP-OES ICP/MS	E64
NIST Tomato leaves 1573 (contd.)	Mn	HNO3	Multimode/ flow through	2 sec at 650 W (0.5 mL coil, 15.4 mL min-1)	F-AAS	E99
	Al, As, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Sb, Se, V, Zn	Aqua regia	Multimode/ LP closed	10 min at 300 W, 5 min at 600 W, 10 min at 480 W	ICP-OES ETV-AAS	E56
	Ca, Fe, K, Mg, Mn, Na, P, S	1. HNO3 2. H2O2 3. HCl	Multimode/ LP closed	1. 30 min at 540 W, 5 min at 0 W 2. 5 min at 0 W, 15 min at 540 W, 5 min at 0 W 3. 10 min at 180 W	ICP-OES	E81
NIST Pine needles 1575	Pb	HNO3, HCl	Multimode/ flow through	18 sec at 700 W (0.4 mL coil, 1.5 mL min-1)	ETV-AAS	E8
	Ba, Ca, K, Mg, Mn, Na, P, S, Zn	1. HNO3 2. H2O2	Multimode/ open	1. 30 min at 540 W 2. 30 min at 540 W	ICP-OES	E42
NIST Pine needles 1575 (contd.)	Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn	A. 1. HNO3 2. H2O2 3. H2O2 B. 1. HNO3 2. H2O2, HF 3. H3BO3	Multimode/ open Multimode/ open (with reflux top)	1. 5 min at 300 W, 600 W until 1 mL remains 2. 30-40 min at 600 W until 1 mL remains 1. 5 min at 300 W, 20-30 min at 600 W reflux not on 2. 30 min at 180 W reflux on 3. 180 W reflux on	FI-ICP-OES	E35
	Al, As, Cr, Cu, Fe, Hg, Mn, Pb, Rb, Sr, Th, U	1. HNO3 2. HF	Multimode/ MP closed	1. Leave overnight, 3 min at 130 W, 6 min at 195 W, 6 min at 260 W, 6 min at 325 W, cool, vent 2. 6 min at 325 W	ICP/MS	E98
	Ca, Cu, Fe, K, Mn, P, Pb	HNO3	Multimode/ HP closed	30 sec at 665 W	F-OES F-AAS ICP-OES	E49
	Hg	HNO3	Multimode/ LP closed	800 W until 75°C, 3 min at 75°C	CV-FANES	E86
NIST Pine needles 1575 (contd.)	As, Ca, Cu, Fe, Mg, Mn, Mo, P, S, Se, U, V, Zn	HNO3, H2O2	Multimode/ MP closed (Max 350 psi - power cutoff at 150 psi)	4 min at 296 W, 8 min at 360 W	F-AAS ICP/MS ICP-OES	E79
	Al, Ba, Ca, Cr, Fe, K, Mg, Mn, Na, P, Zn	1. Aqua regia 2. HBO3	Multimode/ MP closed (evacuated slightly at start)	1. 3 min at 625 W	ICP-OES	E55
	Al, As, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Sb, Se, V, Zn	Aqua regia	Multimode/ LP closed	10 min at 300 W, 5 min at 600 W, 10 min at 480 W	ICP-OES ETV-AAS	E56
	Cd, Co, Fe, Ni, Pb	HNO3, HF	Multimode/ LP closed	1 min at 240 W, cool (x15)	ETV-AAS XRF	E83
NIST Pine needles 1575 (contd.)	Ca, Fe, K, Mg, Mn, Na, P, S	1. HNO3 2. H2O2 3. HCl	Multimode/ LP closed	1. 30 min at 540 W, 5 min at 0 W 2. 5 min at 0 W, 15 min at 540 W, 5 min at 0 W 3. 10 min at 180 W	ICP-OES	E81
	Ca, K, Mg, P	1. HNO3, HClO4 2. HClO4	Multimode/ open (with scrubber)	15 min at 750 W	ICP-OES	E91
	Pb	HNO3	Multimode/ LP closed	3 min at 650 W	ETV-AAS	E3
	Al	HNO3	Multimode/ LP closed	40 sec at 700 W, 10 min at 0 W (vent), 60 sec at 700 W, 10 min at 0 W (vent), 90 sec at 700 W, 5 min at 70 W	ETV-AAS	E63
	Cu	1. HNO3 2. H2O2	Multimode/ LP closed	9.5 min at 540 W	F-AAS	E100
NIST Pine needles 1575 (contd.)	Cu, Fe, Pb	1. HNO3, H2O, H2O2 2. Aqua regia, HF (optional)	Multimode/ open	1. 15 min at 70 W, 15 min at 150 W, 15 min at 180 W, 1 min at 250 W, 1 min at 600 W 2. 15 min at 70 W, 1 min at 600 W	ETV-AAS	E5
	B	HNO3, H2O2	Multimode/ HP closed	2 min at 100W, 2 min at 0 W, 5 min at 250 W, 3 min at 0 W, 5 min at 500 W, 15 min at 250 W	UV-Vis Fluorimetry ICP-OES ICP/MS	E64
	Ca, Fe	HNO3, HCl, HF	Multimode/ MP closed	15 min at 540 W	DCP-OES	E101
	Al, As, Ba, Cr, Cu, Fe, K, Mg, Mn, Ni, P, Pb, Rb, Sr, Zn	1. HNO3 2. HF 3. H2O2	Multimode/ HP closed	1. 5 min at 300 W, 2 min at 600 W, 2 min at 0 W, 3 min at 300 W 2. 5 min at 300 W, 2 min at 600 W, 2 min at 0 W 3. 5 min at 300 W, 2 min at 600 W, 2 min at 0 W, 3 min at 300 W	DCP-OES F-AAS	E96
NIST Corn stalks 8412	Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn	A. 1. HNO3 2. H2O2 3. H2O2 B. 1. HNO3 2. H2O2, HF 3. H3BO3	Multimode/ open Multimode/ open (with reflux top)	1. 5 min at 300 W, 600 W until 1 mL remains 2. 30-40 min at 600 W until 1 mL remains 1. 5 min at 300 W, 20-30 min at 600 W reflux off 2. 30 min at 180 W reflux on 3. 180 W reflux on	FI-ICP-OES	E35
NIST Corn kernel 8413	Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn	A. 1. HNO3 2. H2O2 3. H2O2 B. 1. HNO3 2. H2O2, HF 3. H3BO3	Multimode/ open Multimode/ open (with reflux top)	1. 5 min at 300 W, 600 W until 1 mL remains 2. 30-40 min at 600 W until 1 mL remains 1. 5 min at 300 W, 20-30 min at 600 W reflux not on 2. 30 min at 180 W reflux on 3. 180 W reflux on	FI-ICP-OES	E35
	Pb	HNO3, V2O5	Multimode/ HP closed	90 sec at 460 W	ETV-AAS	E104
	Hg	HNO3	Multimode/ HP closed	90 sec at 600 W	CV-AAS	E105

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