Appendix 1. Biological Reference Materials


Appendix 1.1 Animal tissue and fluid
Matrix Analytes Reagents Cavity/Vessel Conditions Detection Ref.

BCR

Milk powder

63R

 Cd, Cu, Pb HNO3, HClO4, H2SO4

Multimode/

HP closed

 10 min at 85 bar DP-ASV E1

BCR

Milk powder

150

 Hg HNO3

Multimode/

LP closed

 8 min at 140 W, 8 min at 280 W, 4 min at 420 W CV-AAS E2
Cu, Pb HNO3

Multimode/

LP closed

 3 min at 650 W ETV-AAS E3
Cd HNO3, HCl

Multimode/

HP closed

 4 min at 360 W, 10 min at 180 W ETV-AAS E4
Cd, Cu, Fe, Pb

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

 ETV-AAS E5

BCR

Skim milk powder 151

 Hg HNO3

Multimode/

LP closed

8 min at 140 W,

8 min at 280 W,

4 min at 420 W

 CV-AAS E2
Cd HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

 ETV-AAS E4
Cd, Cu, Fe

A.

HNO3, H2O2

B.

HNO3, H2O2

C.

HNO3, HClO4


Multimode/

HP closed

Multimode/

HP closed

Multimode/

HP closed


75 sec at 665 W

1 min at 250 W, 2 min at 0 W,

2 min at 250 W, 2 min at 400 W,

2 min at 600 W

150 sec at 950 W, 60 sec at 0 W,

90 sec at 300 W, 90 sec at 500 W,

90 sec at 700 W, 90 sec at 850 W

 ETV-AAS E6
Hg HNO3

Multimode/

MP closed

8 min at 140 W,

8 min at 280 W,

4 min at 420 W

 CV-AFS E7

BCR

Bovine muscle 184

 Pb HNO3, HCl

Multimode/

flow through

18 sec at 700 W

(0.4 mL coil, 1.5 mL min-1)

 ETV-AAS E8
Cu, Pb HNO3, HClO4, H2SO4

Multimode/

HP closed

 10 min at 85 bar DP-ASV E1
Ni HNO3, HCl

Multimode/

HP closed

4 min at 350 W,

10 min at 140 W

 ETV-AAS E9
Cd, Cu, Fe, Mn, Pb

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

 ETV-AAS E5
Cd HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

 ETV-AAS E4
Ni HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

 ICP-OES E10, E11
Pb HNO3

Multimode/

LP closed

 3 min at 650 W ETV-AAS E3

BCR

Bovine liver

185

 Cd, Pb HNO3, HClO4, H2SO4

Multimode/

HP closed

 10 min at 85 bar DP-ASV E1

BCR

Pig kidney 186

 Pb HNO3, HCl

Multimode/

flow through

18 sec at 700 W

(0.4 mL coil, 1.5 mL min-1)

 ETV-AAS E8
Hg HNO3

Multimode/

LP closed

8 min at 140 W,

8 min at 280 W,

4 min at 420 W

 CV-AFS E7
Ni HNO3, HCl

Multimode/

HP closed

4 min at 350 W,

10 min at 140 W

 ETV-AAS E9
Cd, Cu, Fe, Mn, Pb

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

 ETV-AAS E5
Cd HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

 ETV-AAS E4
Ni HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

 ICP-OES E10, E11

BCR

Whole blood

194

 Pb HNO3

Multimode/

LP closed

15 min at 540 W,

20 min at 420 W

 TXRF E12

BCR

Whole blood

195

 Pb HNO3

Multimode/

LP closed

15 min at 540 W,

20 min at 420 W

 TXRF E12

BCR

Whole blood

196

 Pb HNO3

Multimode/

LP closed

15 min at 540 W,

20 min at 420 W

 TXRF E12

BCR

Human hair

397

 Hg HNO3

Multimode/

LP closed

8 min at 140 W,

8 min at 280 W,

4 min at 420 W

 CV-AFS E7
Cd, Hg, Pb, Zn

A.

1. HNO3

2. HNO3

3. H2O2

4. H2O

B.

1. HNO3, HCl

2. HNO3, HCl

3. H2O2

4. H2O


Single mode/

open




Single mode/

open


1. 5 min at 10 W,

10 min at 30 W,

10 min at 60 W

2. 10 min 60 W

3. 5 min at 60 W

4. 5 min at 50 W

1. 5 min at 40 W,

10 min at 50 W

2. 20 min 54 W

3. 5 min at 40 W

4. 5 min at 50 W

ICP-OES

ICP-MS

HPLC-ICP-MS

 E13

BI

Control blood for metals

620401

 Hg(II), methylmercury, mercurescein, phenylmercury

1. HCl, KBr, KBrO3

2. KMnO4

3. NaBH4, NaOH

Single mode/

flow through

1. 25.4 sec at 70 W

(14-16 mL min-1)

 CV-AAS E14

BI

Control metals in urine Lanonorm M1

 Cd HNO3

Multimode/

LP closed

 3 min at 650 W ETV-AAS E3
Hg

1. K2Cr2O7, HNO3, KBr, KBrO3

2. HCl

Single mode/

flow through

1. Offline

2. 65 sec at 90 W (8.5 mL min-1)

 CV-AAS E15

BI

Control metals in urine

Lanonorm M2

 Hg

1. K2Cr2O7, HNO3, KBr, KBrO3

2. HCl

Single mode/

flow through

1. Offline

2. 65 sec at 90 W

(8.5 mL min-1)

 CV-AAS E15

BI

Control metals in urine

Lanonorm M3

 Cd, Pb HNO3

Multimode/

LP closed

 3 min at 650 W ETV-AAS E3

BI

Control blood for metals

OSSD-20/21

 Hg HNO3

Multimode/

HP closed

 70 sec at 600 W CV-AAS E16

BRL

Urine metals level II

39202

 Hg

1. K2Cr2O7, HNO3, KBr, KBrO3

2. HCl

Single mode/

flow through

1. Offline

2. 65 sec at 90 W (8.5 mL min-1)

 CV-AAS E15

IAEA

Milk powder

A11

 I HNO3, N2H4

Multimode/

HP closed

 35 sec at 675 W NAA E17
Se

1. HNO3

2. H2SO4, HClO4

3. H2O

Single mode/

open

1. 15 min at 45 W,

10 min at 75 W

2. 10 min at 90 W,

35 min at 120 W

3. 8 min at 120 W

 ETV-AAS E18
Cu, Fe, Ni, Zn

A.

1. HNO3

2. H2O2



B.

1. HNO3, H2SO4

2. HNO3

3. HNO3

C.

HNO3


Multimode/

LP closed




Single mode/

open



Single mode/

open


1. 3 min at 280 W,

2-5 min at 0 W,

3 min at 420 W,

2-5 min at 0 W,

2 min at 560 W

2. 2 min at 260 W

1. 5 min at 80 W

2. 5 min at 60 W, 5 min at 80 W

3. 5 min at 80 W

(for fat rich foods)

10 min at 80 W

(for lower fat foods)

F-AAS

ETV-AAS

HG-AAS

 E19
Al HNO3

Multimode/

HP closed

1 min at 150 W, 30 min at 0 W,

1 min at 450 W

 ICP-OES E20

IAEA

Animal blood

A13

 Ca, Cu, Fe, K, Ni, P, Pb, Rb, S, Se, Sr, Zn HNO3

Multimode/

LP closed

15 min at 540 W,

20 min at 420 W

 TXRF E12

IAEA

Whey powder

A155

 Al HNO3

Multimode/

HP closed

1 min at 150 W,

30 min at 0 W,

1 min at 450 W

 ICP-OES E20

IAEA

Animal muscle

H4

 I HNO3, N2H4

Multimode/

HP closed

 35 sec at 675 W NAA E17
Se

1. HNO3

2. H2SO4, HClO4

3. Water

Single mode/

open

1. 15 min at 45 W,

10 min at 75 W

2. 10 min at 90 W,

35 min at 120 W

3. 8 min at 120 W

 ETV-AAS E18
Al, As, Ba, Ca, Cd, Ce, Cr, Cs, Cu, Fe, La, Li, Mg, Mn, Mo, Ni, Rb, Sb, Se, Sr, Tl, V, Y, Zn HNO3

Multimode/

HP closed

 75 sec at 350 W ICP/MS E21
Al, Ca, Cu, Fe, K, Mg, Mn, Na, P, Sr, Zn HNO3

Multimode/

LP closed

10 min at 70 psi

15 min at 450 W

 ICP-OES E22
Al, Cd, Co, Cu, Fe, Mg, Mn, Mo, Rb, Sr, Zn HNO3

Multimode/

LP closed

 2 min at 600 W ICP/MS E23

IAEA

Horse kidney

H8

 Al, Cd, Co, Cu, Fe, Mg, Mn, Mo, Rb, Sr, Zn HNO3

Multimode/

LP closed

 2 min at 600 W ICP/MS E23

IAEA

Horse kidney

H8

(contd.)

 Ca, Cd, Cu, Fe, K, Mg, Mn, Na, P, Sr, Zn, Residual carbon

A.

1. HNO3, H2SO4

2. H2O2(x16)

3. HNO3 or

HNO3, HCl or

NH3 (aq)

4. NH4EDTA

B.

HNO3


Single mode/

open




Multimode/

MP closed


1. 4 min at 30 W,

4 min at 120 W

2. 1 min at 120 W(x16)

3. 4 min at 30 W


2 min at 20 psi,

5 min at 40 psi,

2 min at 60 psi,

2 min at 80 psi,

2 min at 100 psi,

2 min at 120 psi,

2 min at 140 psi,

15 min at 160 psi

 ICP-OES E24
Ca, Cd, Fe, Mg, Zn

A.

HNO3

B.

HNO3


Multimode/

stopped flow

Multimode/

LP closed


2 min at 300 W,

1 min at 0 W,

3 min at 150 W

30 min at 420 W

F-AAS

ICP-OES

 E25

KL

Heavy metal urine control

0140

 Hg HNO3

Multimode/

HP closed

 70 sec at 600 W CV-AAS E16

ISS

Green algae

MMM-2

 Cr, Co, Mn, Ni, Pb HNO3, H2O2

Multimode/

LP closed

 6 min at 700 W

F-AAS

ICP-OES

 E26

NIES

Human hair

5

 Ca, Cu, Fe, Mg, Mn, Zn HNO3, HClO4, HCl, HF

Multimode/

MP closed

 14 min intermittent 200 W F-AAS E27
Cd, Co, Cu, Ni, Pb HNO3, HCl, HClO4, HF

Multimode/

MP closed

5 min at 200 W (water load),

3.5 min at 200 W (no load)

ICP-OES

ETV-ICP

 E28

NIES

Human hair

5

(contd.)

 Cu, Pb, Residual carbon

A.

HNO3

B.

HNO3, H2O2

C.

HNO3, H2O2

D.

1. HNO3 or H2O2

2. HNO3 vapor


Multimode/

LP closed

Multimode/

LP closed

Multimode/

MP closed

Multimode/

MP closed


0.5- 5 min at 550 W

(with water ballast)

0.5-5 min at 550 W

(with water ballast)

15-60 sec at 550 W

(with water ballast)

10 min at 550 W

(with water ballast)

ETV-AAS
DP-ASV

Coulometry

 E29
Cr, Hg, Se

1. HNO3

2. H2O2

Multimode/

HP closed

 1. 3 min at 450 W Radiochemical E30

NIST

Human serum

909

 Cr, Cu, Fe, K, Li, Mg, Mn, Mo, Na, P, Pb, S, Sr, Zn

1. HNO3

2. HClO4

Multimode/

MP closed

 1. 1 min at 90 W, 1 min at 0 W (x5) ICP-OES E31
Ni HNO3, HCl

Multimode/

HP closed

4 min at 350 W,

10 min at 140 W

 ETV-AAS E9

NIST

Human serum

909

(contd.)

 Ca, Cr, Cu, Fe, K, Mn, Ni, P, Pb, Rb, S, Se, Sr, Zn HNO3

Multimode/

LP closed

15 min at 540 W,

20 min at 420 W

 TXRF E12

NIST

Albumin

926

 No analysis HNO3

Multimode/

LP closed

 Varied

Temperature

Pressure

 E32

NIST

Milk powder

1549

 I HNO3, N2H4

Multimode/

HP closed

 35 sec at 675 W NAA E17
Ca, Mg

1. HNO3, H2SO4, H2O2, H2O

2. H2O2

3. HNO3, H2O

4. HNO3, H2O

Single mode/

stopped flow

1. 3 min at 20 W (condenser on), 2 min at 56 W (condenser on),

10 min at 80 W (condenser off)

2. 3 min at 116 W (condenser on)

3. 2 min at 80 W (condenser on)

4. 2 min at 80 W (condenser on)

 ICP-OES E33
Cr, Cu, Ni, Pb, Sn, Zn HNO3, H2O2

Multimode/

LP closed

 3 min at ? W

ETV-AAS

F-AAS

 E34

NIST

Milk powder

1549

(contd.)

 Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

 FI-ICP-OES E35
Ca, Cd, Cu, Fe, K, Mg, Mn, Na, P, Sr, Zn, Residual carbon

A.

1. HNO3, H2SO4

2. H2O2(x16)

3. HNO3 or

HNO3, HCl or

NH3 (aq)

4. NH4EDTA

B.

HNO3


Single mode/

open




Multimode/

MP closed


1. 4 min at 30 W,

4 min at 120 W

2. 1 min at 120 W(x16)

3. 4 min at 30 W


2 min at 20 psi,

5 min at 40 psi,

2 min at 60 psi,

2 min at 80 psi,

2 min at 100 psi,

2 min at 120 psi,

2 min at 140 psi,

15 min at 160 psi

 ICP-OES E24
Zn, Residual carbon

A.

HNO3, H2SO4

B.

HNO3

or HNO3, H2SO4

or HNO3, H2O2

or HNO3, H2SO4,

H2O2

C.

HNO3, H2O2, H2SO4


Multimode/

LP closed

Multimode/

MP closed



Single mode/

open


2 min at 130 W, 4 min at 260 W, 2 min at 390 W, 2 min at 520 W, 12 24 or 72 min at 650 W

2 min at 20 psi, 5 min at 40 psi,

2 min at 60 psi, 2 min at 80 psi,

2 min at 100 psi, 2 min at 120 psi, 2 min at 140 psi,

15 min at 160 psi

4 min at 30 W, 6 9 or 16 min at 120 W, 10 20 or 30 min at 205 W

 ICP-OES E36, E37

NIST

Brewers yeast

1569

 Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

 FI-ICP-OES E35

NIST

Bovine liver 1577

 Zn, Cd HNO3

Multimode/

open

 8 min at 200 W FI-F-AAS E38
Ca, Fe, Mg, Zn HNO3

Multimode/

flow through

100 sec at 525 W

(10 mL coil,

6 mL min-1)

 F-AAS E39
Ag, Al, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, P, Pb, S, Sb, Se, Si, Sr, Ti, Tl, V, Zn

1. HNO3

2. HClO4

Multimode/

MP closed

 1. 1 min at 90 W, 1 min at 0 W (5 times) ICP-OES E31
Pb HNO3, HCl

Multimode/

flow through

18 sec at 700 W

(0.4 mL coil,

1.5 mL min-1)

 ETV-AAS E8
Se

1. HNO3

2. H2SO4, HClO4

3. H2O

Single mode/

open

1. 15 min at 45 W,

10 min at 75 W

2. 10 min at 90 W,

35 min at 120 W

3. 8 min at 120 W

 ETV-AAS E18
I

1. HNO3

2. N2H4

Multimode/

HP closed

 1. 35 sec at 675 W NAA E17
Cd HNO3

Multimode/

LP closed

 2 min on defrost three times ETV-AAS E40
Hg HNO3

Multimode/

LP closed

8 min at 140 W,

8 min at 280 W,

4 min at 420 W

 CV-AAS E2

NIST

Bovine liver

1577

(contd.)

 Na

1. HNO3

2. HNO3, H2SO4

3. H2O2

Multimode/

LP closed

1. 1 min at 630 W

2. 5 min at 273 W,

20 min at 189 W (2-5 samples) or

4 min at 630 W,

8 min at 378 W

(6-12 samples)

 F-AAS E41
Ba, Ca, K, Mg, Mn, Na, P, S, Zn

1. HNO3

2. H2O2

Multimode/

open

1. 30 min at 540 W

2. 30 min at 540 W

 ICP-OES E42
Ca, Cu, Fe, Mg, Mn, Zn HNO3, HClO4, HCl, HF

Multimode/

MP closed

 14 min at 200 W F-AAS E27
Residual amino acids HNO3

Multimode/

LP closed

 Varied Fluorimetry E43
- HNO3

Multimode/

LP closed

 Varied

Temperature

Pressure

 E32
Al, Ba, Ca, K, Mg, Mn, Na, P, S, Si, Zn

A.

1. HNO3

2. H2O2

3. H2O2

B.

1. HNO3

2. H2O2, HF

3. H3BO3


Multimode/

open


Multimode/

open

(with reflux top)


1. 5 min at 300 W,

600 W until 1 mL remains

2. 30-40 min at 600 W

until 1 mL remains

1. 5 min at 300 W,

20-30 min at 600 W reflux not on

2. 30 min at 180 W reflux on

3. 180 W reflux on

 FI-ICP-OES E35
Se HNO3

Multimode/

LP closed

(1.5 h at 60°C on hotplate)

4 min at 287 W

 ETV-AAS E44
Residual organic species, Cu, Zn HNO3

Multimode/

LP closed

5 min at 132 W,

5 min at 207 W

(additional 3 min at 230 W

if temp not at 180°C for 100 sec)

Voltammetry

LC

UV-Vis

 E45
As, Co, Cu, Se, Zn HNO3, HClO4

Multimode/

open

 600 W until first signs of HClO4 fumes

F-AAS

NAA

 E46, E47, E48
Ca, Cd, Cu, Fe, K, Mg, Mn, Na, Zn HNO3

Multimode/

HP closed

 30 sec at 665 W

F-OES

F-AAS

ICP-OES

 E49
Cd, Cr, Cu, Fe, Pb, Zn HNO3, H2SO4

Multimode/

LP closed

 60 sec at 700 W F-AAS E50

NIST

Bovine liver 1577

(contd.)

 Cd, Cr, Cu, Fe, Pb, Zn HNO3, H2SO4

Multimode/

open

10 sec at 70 W,

180 sec at 0 W (x6)

 F-AAS E51
Al, As, Ba, Ca, Cd, Ce, Cr, Cs, Cu, Fe, La, Li, Mg, Mn, Mo, Ni, Rb, Sb, Se, Sr, Tl, V, Y, Zn HNO3

Multimode/

HP closed

 75 sec at 350 W ICP/MS E21
B, Ca, Fe, K, Mg, Mn, Na, Ni, P HNO3, HClO4

Multimode/

open

 20 min at 600 W ICP-OES E52
Fe HNO3, H2O2

Multimode/

LP closed

 15 min at 150 W F-AAS E53
Cu, Fe, Mn, Zn HNO3

Multimode/

LP closed

 8 min at 150 W

F-AAS

ETV-AAS

SIMAAC

 E54
Ca, Cu, Fe, K, Mg, Mn, Na, P , S, Zn HNO3

Multimode/

LP closed

10 min at 70 psi,

15 min at 450 W

NAA

ICP-OES

 E22
Al, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, P, Zn

1. Aqua regia

2. HBO3

Multimode/

MP closed

(evacuated slightly at start)

 1. 3 min at 625 W ICP-OES E55
As, Be, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Sb, Se, V, Zn Aqua regia

Multimode/

LP closed

10 min at 300 W,

5 min at 600 W,

10 min at 480 W

ICP-OES

ETV-AAS

 E56
Cu, Mn, Zn

1. HNO3

2. HClO4

Multimode/

LP closed

 Varied SPC-IC E57
Al, Ba, Ca, Cd, Cu, Fe, Mg, Pb, Zn

A.

Aqua regia

B.

Aqua regia


Multimode/

stopped flow

Multimode/

open


2 min at 720 W

2 min at 720 W,

2 min at 0 W,

2 min at 720 W,

2 min at 0 W,

2 min at 720 W

(with water load)

 ICP-OES E58
Al, Cd, Co, Cu, Fe, Mg, Mn, Mo, Rb, Sr, Zn HNO3

Multimode/

LP closed

 2 min at 600 W ICP/MS E23

NIST

Bovine liver

1577

(contd.)

 Cu, Fe HNO3

Multimode/

LP closed

 3 min at 650 W ETV-AAS E3
Residual carbon HNO3

Multimode/

HP closed

5 min at 500 W

(200 bar)

Total carbon analyzer

Carbon dioxide coulometer

 E59
As

1. HNO3, H2SO4

2. H2O2

3. H2O2

Multimode/

LP closed

1. 4 min at 100 W, cool to RT,

6 min at 325 W, cool to RT

2. 6 min at 325 W, cool to RT,

6 min at 650 W, cool to RT

3. 1 h at 90°C (waterbath, open)

 HG-AAS E60
Residual carbon HNO3

Multimode/

HP closed

 4 min at 90 W

Total carbon analyzer

Carbon dioxide coulometer

 E61
Ca, Cd, Cu, Fe, K, Mg, Mn, Na, P, Sr, Zn, Residual carbon

A.

1. HNO3, H2SO4

2. H2O2(x16)

3. HNO3 or

HNO3, HCl or

NH3 (aq)

4. NH4EDTA

B.

HNO3


Single mode/

open




Multimode/

MP closed


1. 4 min at 30 W,

4 min at 120 W

2. 1 min at 120 W(x16)

3. 4 min at 30 W


2 min at 20 psi,

5 min at 40 psi,

2 min at 60 psi,

2 min at 80 psi,

2 min at 100 psi,

2 min at 120 psi,

2 min at 140 psi,

15 min at 160 psi

 ICP-OES E24
Ag, Al, As, Cd, Co, Cu, Fe, Hg, Mn, Mo, Pb, Rb, Sb, Se, Sr, Tl, V, Zn HNO3

Multimode/

HP closed

 2 min at 300 W ICP/MS E62

NIST

Bovine liver

1577

(contd.)

 Al HNO3

Multimode/

LP closed

40 sec at 700 W,

10 min at 0 W (vent),

60 sec at 700 W,

10 min at 0 W (vent),

90 sec at 700 W,

5 min at 70 W

 ETV-AAS E63
Cd, Cu, Fe, Mn, Pb

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

 ETV-AAS E5
B HNO3, H2O2

Multimode/

HP closed

2 min at 100W,

2 min at 0 W,

5 min at 250 W,

3 min at 0 W,

5 min at 500 W,

15 min at 250 W

UV-Vis

Fluorimetry

ICP-OES

ICP/MS

 E64

NIST

Bovine liver

1577

(contd.)

 Al HNO3

Multimode/

LP closed

40 sec at 700 W,

10 min at 0 W (vent),

60 sec at 700 W,

10 min at 0 W (vent),

90 sec at 700 W,

5 min at 70 W

 ETV-AAS E63
Cd, Cu, Fe, Mn, Pb

1. HNO3, H2O, H2O2

2. Aqua regia, HF

(optional)

Multimode/

open

1. 15 min at 70 W,

15 min at 150 W,

15 min at 180 W,

1 min at 250 W,

1 min at 600 W

2. 15 min at 70 W,

1 min at 600 W

 ETV-AAS E5
B HNO3, H2O2

Multimode/

HP closed

2 min at 100W,

2 min at 0 W,

5 min at 250 W,

3 min at 0 W,

5 min at 500 W,

15 min at 250 W

UV-Vis

Fluorimetry

ICP-OES

ICP/MS

 E64
Cd HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

 ETV-AAS E4
Se HNO3, HClO4

Multimode/

open

 Not reported Fluorimetry E65
Fe, Mn, Zn HNO3

Multimode/

flow through

 2 min at ? W

F-AAS

ICP-OES

ICP/MS

 E66
Ca, Cu, Fe, Mg, Mn, K, Na, Zn

1. HNO3

2. HNO3

3. H2O2

Single mode/

open

1. 10 min at 60 W,

5 min at 80 W

2. 5 min at 70 W

3. 3 min at 60 W

 F-AAS E67
Se HNO3

Multimode/

open

2 min on MedHi,

2 min at 0 W (x3)

 ETV-AAS E68

NIST

Bovine serum

1598

 Al, As, Cd, Cr, Cu, Mn, Mo, Sb, V HNO3

Multimode/

LP closed

 8 min at 164 W NAA E69

NIST

Freeze dried

urine

2670

 Residual amino acids HNO3

Multimode/

LP closed

 Varied Fluorimetry E43
Hg

1. K2Cr2O7, HNO3, KBr, KBrO3

2. HCl

Single mode/

flow through

1. Offline

2. 65 sec at 90 W (8.5 mL min-1)

 CV-AAS E15
Ni HNO3, HCl

Multimode/

HP closed

4 min at 350 W,

10 min at 140 W

 ETV-AAS E9

NIST

Freeze dried urine

2670

(contd.)

 Cd HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

 ETV-AAS E4
Ca, Cd, Cu, K, Mg, Mn, Na, Residual carbon

A.

1. HNO3, H2SO4

2. H2O2(x16)

3. HNO3 or

HNO3, HCl or

NH3 (aq)

4. NH4EDTA

B.

HNO3


Single mode/

open




Multimode/

MP closed


1. 4 min at 30 W,

4 min at 120 W

2. 1 min at 120 W(x16)

3. 4 min at 30 W


2 min at 20 psi,

5 min at 40 psi,

2 min at 60 psi,

2 min at 80 psi,

2 min at 100 psi,

2 min at 120 psi,

2 min at 140 psi,

15 min at 160 psi

 ICP-OES E24
Ca, Mg

1. HNO3, H2SO4, H2O2, H2O

2. H2O2

3. HNO3, H2O

4. HNO3, H2O

Single mode/

stopped flow

1. 3 min at 20 W (condenser on), 2 min at 56 W (condenser on),

10 min at 80 W (condenser off)

2. 3 min at 116 W (condenser on)

3. 2 min at 80 W (condenser on)

4. 2 min at 80 W (condenser on)

 ICP-OES E33
Ni HNO3, HCl

Multimode/

HP closed

4 min at 360 W,

10 min at 180 W

 ICP-OES E11

NIST

Bovine serum

8419

 Ca, Cu, Fe, K, Mg, Na HNO3

Multimode/

HP closed

 30 sec at 665 W

F-OES

F-AAS

ICP-OES

 E49

SERONORM

Trace elements in blood

B115

 Hg

1. K2Cr2O7, HNO3, KBr, KBrO3

2. HCl

Single mode/

flow through

1. Offline

2. 65 sec at 90 W (8.5 mL min-1)

 CV-AAS E15

SERONORM

Trace elements in urine

116

 As

1. HNO3, H2SO4

2. H2O2

3. H2O2

Multimode/

LP closed

1. 4 min at 100 W, cool to RT,

6 min at 325 W, cool to RT

2. 6 min at 325 W, cool to RT,

6 min at 650 W, cool to RT

3. 1 h at 90°C (waterbath, open)

 HG-AAS E60

SERONORM

Trace elements in blood

#904

 Pb HNO3, HCl

Multimode/

flow through

18 sec at 700 W

(0.4 mL coil, 1.5 mL min-1)

 ETV-AAS E8

SERONORM

Trace elements in blood

#905

 Hg(II), methylmercury, mercurescein, phenylmercury

1. HCl, KBr, KBrO3

2. KMnO4

3. NaBH4, NaOH

Single mode/

flow through

1. 25.4 sec at 70 W

(14-16 mL min-1)

 CV-AAS E14

SERONORM

Trace elements in blood

#906

 Hg(II), methylmercury, mercurescein, phenylmercury

1. HCl, KBr, KBrO3

2. KMnO4

3. NaBH4, NaOH

Single mode/

flow through

1. 25.4 sec at 70 W

(14-16 mL min-1)

 CV-AAS E14

SERONORM

Trace elements in urine

# 009024

 Bi, Hg

1. HCl, KBr, KBrO3

2. NaBH4, NaOH

Single mode/

flow through

1. 65 sec at 90-120 W

(8.5 mL min-1)

CV-AAS

HG-AAS

 E70, E71

SERONORM

Trace elements in blood

#010010

 Hg(II), methylmercury, mercurescein, phenylmercury

1. HCl, KBr, KBrO3

2. KMnO4

3. NaBH4, NaOH

Single mode/

flow through

1. 25.4 sec at 70 W

(14-16 mL min-1)

 CV-AAS E14

SERONORM

Trace elements in blood

#010011

 Hg(II), methylmercury, mercurescein, phenylmercury

1. HCl, KBr, KBrO3

2. KMnO4

3. NaBH4, NaOH

Single mode/

flow through

1. 25.4 sec at 70 W

(14-16 mL min-1)

 CV-AAS E14

SERONORM

Trace elements in blood

#010012

 Hg(II), methylmercury, mercurescein, phenylmercury

1. HCl, KBr, KBrO3

2. KMnO4

3. NaBH4, NaOH

Single mode/

flow through

1. 25.4 sec at 70 W

(14-16 mL min-1)

 CV-AAS E14

SERONORM

Trace elements in blood

#205052

 Hg(II), methylmercury, mercurescein, phenylmercury

1. HCl, KBr, KBrO3

2. KMnO4

3. NaBH4, NaOH

Single mode/

flow through

1. 25.4 sec at 70 W

(14-16 mL min-1)

 CV-AAS E14

SERONORM

Trace elements in blood

#203056

 Hg(II), methylmercury, mercurescein, phenylmercury

1. HCl, KBr, KBrO3

2. KMnO4

3. NaBH4, NaOH

Single mode/

flow through

1. 25.4 sec at 70 W

(14-16 mL min-1)

 CV-AAS E14

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